In this paper, we obtained the first brain map of a complete fruit fly head at 10 micron isotropic resolution, the highest ever reported by MR for a complete head. Using two complementary imaging sequences revealed the superior power of DWI to dissect the brain architecture at close to cellular resolution.
Dynamic nuclear polarization (DNP) coupled with solid state NMR can provide orders of magnitude enhancement to normally weak NMR signals, thereby enabling the study of inherently dilute proteins such as membrane proteins. Here we demonstrate a new approach to obtain DNP signal enhancements of membrane proteins by utilizing spin labeled lipids as the polarization agents. This strategy results in more than 2x in signal enhancements of a membrane protein when compared to standard DNP sample preparation techniques.
An overpressure furnace capable of developing high current density in significant-sized coils (up to 15 cm diameter and 50 cm long) has been brought into commission. The furnace is enabling reaction of solenoids made out of Bi-2212 destined for tests of NMR quality magnets at proton frequencies greater than 1 GHz.
CrgA, a key Mycobacterium tuberculosis cell division protein that recruits five other proteins to the cell division apparatus has been structurally characterized using oriented sample and magic angle spinning solid state NMR. The protein has two transmembrane helices and an intrinsically disordered N-terminus. Binding sites have been identified for it's binding partners. Evaluating these binding sites may lead to effective drugs for either promoting and inhibiting cell division, both of which are of prime interest for the treatment of tuberculosis.
13C NMR when used in metabolomics 1. Provides better peak list for database matching and spectral annotation, 2. Provides better group separation and loadings annotation when using multivariate statistical analysis, and 3. Prevents possible misidentification of metabolites.
By coupling selective band excitation of metabolites with high magnetic fields, relaxation-enhanced 1H MR spectroscopy can be performed in living specimen and patients to achieve high sensitivity over very short acquisition times for the examination of cellular dysfunction. This sensitivity can be used to evaluate otherwise inaccessible metabolites or regions of the proton spectral regime and can be used to probe cell-specific environments, such as neurons versus astrocytes, that may undergo differential changes during dysregulation.
Targeted theranostic nanovehicles are capable of targeting cerebrovascular amyloid associated with Alzheimer’s Disease and serving as early diagnostic and therapeutic agents across multiple imaging modalities. Assessed in animal models at 21.1 T, these nanovehicles were loaded with gadolinium-based magnetic resonance imaging (MRI), iodine-based single photon emission computerized tomography (SPECT) or fluorescent contrast agents as well as anti-inflammatory and anti-amyloidogenic pharmaceuticals to demonstrate targeted enhancement and treatment in cerebral amyloid angiopathy.
A new non-Brownian model of anomalous translational diffusion in nervous tissue is introduced and applied to the brain. This model provides new fractional order parameters of diffusion, entropy, waiting time and jump length that represent unique markers of morphology in neural tissue.
Sodium MRI techniques point to better cancer treatments.
This MagLab biophysicist is working on an HIV vaccine.