13C NMR when used in metabolomics 1. Provides better peak list for database matching and spectral annotation, 2. Provides better group separation and loadings annotation when using multivariate statistical analysis, and 3. Prevents possible misidentification of metabolites.
Ten years ago the 900 Ultra-Wide Bore magnet became available to an international user community for Nuclear Magnetic Resonance spectroscopy and Magnetic Resonance Imaging at the National High Magnetic Field Lab. Since then 69 publications have been published from this instrument spanning many disciplines and the number of publications per year continues to increase with 26 in just the past 18 months demonstrating that state of the art data continues to be collected on this superb magnet.
Biomedical researchers have a unique tool to investigate a variety of living and excised specimen with the MagLab’s 21.1 T 900-MHz ultra-widebore (105-mm) vertical magnet. However, there are challenges to performing research in a high-field vertical magnet, which have been addressed by a NHMFL-led team of international scientists working to make very high field or ultra high field MRI more flexible. This team has constructed a tunable sliding ring transmit/receive volume coil for 900-MHz hydrogen MRI that provides the uniformity and sensitivity for high resolution and functional imaging of living samples while accommodating unique excised samples to improve characterization and throughput. This new design incorporates the apparatus necessary for maintaining animals in a vertical position while providing remote tuning and sample flexibility beyond most available coils.
Dynamic nuclear polarization (DNP) coupled with solid state NMR can provide orders of magnitude enhancement to normally weak NMR signals, thereby enabling the study of inherently dilute proteins such as membrane proteins. Here we demonstrate a new approach to obtain DNP signal enhancements of membrane proteins by utilizing spin labeled lipids as the polarization agents. This strategy results in more than 2x in signal enhancements of a membrane protein when compared to standard DNP sample preparation techniques.
Scientists have discovered and characterized an unusual, complex natural product produced in worms, a finding that suggests a whole body of discoveries awaits.
Using a novel combination of techniques, scientists researching the COPII protein created a pseudo-atomic model of the COPII cage, gaining a better understanding of how its 96 subunits fit together.
The MagLab’s 21-tesla FT-ICR magnet can identify human proteins far more efficiently than commercial instruments, a boon for medical research.
Scientists analyzing maize affected by southern leaf blight determine the molecular structures of so-called “death acids.”
The MagLab’s AMRIS facility has recently implemented dissolution DNP technology. The system utilizes a 5 T magnet in which samples are cooled to 14,000 gain in SNR on dissolution and injection into our 4.7T MRI/S scanner.
Targeted theranostic nanovehicles are capable of targeting cerebrovascular amyloid associated with Alzheimer’s Disease and serving as early diagnostic and therapeutic agents across multiple imaging modalities. Assessed in animal models at 21.1 T, these nanovehicles were loaded with gadolinium-based magnetic resonance imaging (MRI), iodine-based single photon emission computerized tomography (SPECT) or fluorescent contrast agents as well as anti-inflammatory and anti-amyloidogenic pharmaceuticals to demonstrate targeted enhancement and treatment in cerebral amyloid angiopathy.