12 May 2015
An approach for dynamic nuclear polarization of membrane proteins
Dynamic nuclear polarization (DNP) coupled with solid state NMR can provide orders of magnitude enhancement to normally weak NMR signals, thereby enabling the study of inherently dilute proteins such as membrane proteins. Here we demonstrate a new approach to obtain DNP signal enhancements of membrane proteins by utilizing spin labeled lipids as the polarization agents. This strategy results in more than 2x in signal enhancements of a membrane protein when compared to standard DNP sample preparation techniques.
Details
- Research Area: Biochemistry, Biology
- Research Initiatives: Life
- Facility / Program: EMR, NMR/MRI
- Year: 2015