Overhauser Dynamic Nuclear Polarization

Overhauser DNP has been demonstrated to increase the sensitivity of NMR experiments by one order of magnitude at fields up to 9.4 teslas (T) (corresponding to a NMR proton frequency of 400 MHz).

The increased sensitivity can lead to additional information about the samples being studied, usually a mixture of molecules of interest, and drastically reduce experimental acquisition times. Our group is developing an Overhauser DNP instrument at 14.1 T (corresponding to a NMR proton frequency of 600 MHz).

In short, DNP uses microwave radiation to transfer polarization from stable radicals to NMR active nuclei in molecules of interest thereby increasing the number of polarized nuclear spins tremendously and large signal to noise ratios are observed.

A liquid state DNP NMR, referred to as Overhauser DNP system is being developed at 14.1 T (600 MHz NMR proton frequency, 395 GHz EPR electron frequency). Dynamic nuclear polarization applicable to liquid samples has never been performed at such high fields. This instrument, once completed, would be the first of its kind, potentially reaching unprecedented sensitivity at very high resolution. At this point it will become part of the MagLab user program.

This instrument is currently in its development stage at the Tallahassee location. This system shares a gyrotron, the 395 GHz microwave source, with the MAS DNP NMR user instrument.

This instrument development effort is led by the EMR Facility.

The following spectrometers are available for this technique:

Contacts

Video: Overhauser DNP at the MagLab

Last modified on 8 January 2016