Solution State Capabilities & Techniques

A list of solution state capabilities and techniques available at the MagLab. Please click on the links for more details.

In a collaboration with Agilent we have developed a probe with extraordinary sensitivity for solution NMR studies of chemicals produced by biological organisms. This probe, which now operates at UF’s AMRIS NMR facility, is based on high temperature superconductor (HTS) technology. It is the first HTS probe to use a 1.5-mm sample tube, and also the first HTS probe to have triple resonance capability, allowing it to be used for mass-limited proteins as well as smaller molecules.

The MagLab has spectrometers that can be used for metabolomics studies (see list below), especially the spectrometers equipped with cryoprobe, which provide higher sensitivity. Some actively developing areas include biomarker identification, automatic mixture analysis, 13C detection, etc. The MagLab is also affiliated with the Southeast Center for Integrated Metabolomics at the University of Florida, which provides state-of-the-art metabolomics services to users in all areas of biomedical and biological sciences.

Overhauser DNP has been demonstrated to increase the sensitivity of NMR experiments by one order of magnitude at fields up to 9.4 teslas (T) (corresponding to a NMR proton frequency of 400 MHz).

All the solution NMR spectrometers can be used for Protein Structure and Dynamics studies. The higher field and cryoprobe provide higher sensitivity. Bruker Topspin and Agilent VnmrJ software are used for data collection.