Metabolomics

The MagLab has spectrometers that can be used for metabolomics studies (see list below), especially the spectrometers equipped with cryoprobes which provide higher sensitivity. Some actively developing areas include biomarker identification, automatic mixture analysis, and deuterium and carbon-13 isotope tracer experiments. Technology development for metabolomics is supported by the NIH-funded National Resource for Advanced NMR Technology, RM1 GM148766. Please visit the Resource webpage for information on the development of NMR probe technology for metabolomics and probe capabilities or to start a collaboration. The MagLab is also affiliated with the Southeast Center for Integrated Metabolomics at the University of Florida, which provides state-of-the-art metabolomics services to users in all areas of biomedical and biological sciences.

Details

Available Spectrometers

The following instruments at AMRIS facility are available for this technique:

800 MHz 63 mm NMR Magnet #3 (Gainesville)
800 MHz 54 mm Cryo Bruker Avance III (Gainesville)
600 MHz 51 mm NMR Magnet (Gainesville)

The following instruments at NMR facility are available for this technique:

600 MHz 52 mm NMR Magnet (Tallahassee)

Available Probes

The following probes are available for this technique:

800 MHz TCI solution cryoprobe (Bruker Z44909)
600 MHz 1.5 mm 1H–13C all-HTS probe with PFG (NHMFL-Varian/Agilent)

What's happening at the MagLab tody?

Explore our magnet schedule to see what exciting research is happening on our stellar fleet of instruments right now.

Related publications

J.N. Thomas, T.L. Johnston, I.M. Litvak, V. Ramaswamy, M.E. Merritt, J.R. Rocca, A.S. Edison, W.W. Brey, Implementing High Q-Factor HTS Resonators to Enhance Probe Sensitivity in 13C NMR Spectroscopy, J Phys Conf Ser 2323 (2022) 012030. https://doi.org/10.1088/1742-6596/2323/1/012030.
D.S. Wishart, L.L. Cheng, V. Copie, A.S. Edison, H.R. Eghbalnia, J.C. Hoch, G.J. Gouveia, W. Pathmasiri, R. Powers, T.B. Schock, L.W. Sumner, M. Uchimiya, NMR and Metabolomics-A Roadmap for the Future, Metabolites 12 (2022) 678. https://doi.org/10.3390/metabo12080678.
Ragavan, M.E. Merritt, Nuclear Magnetic Resonance Measurement of Metabolic Flux Using ¹³C and ¹H Signals, in: S.K. Bhattacharya (Ed.), METABOLOMICS: METHODS AND PROTOCOLS, Humana Press Inc, Totowa, 2019: pp. 29–40. https://doi.org/10.1007/978-1-4939-9488-5_3.
A.S. Edison, A. Le Guennec, F. Delaglio, E. Kupce, Practical Guidelines for ¹³C-Based NMR Metabolomics, in: G. a. N. Gowda, D. Raftery (Eds.), NMR-BASED METABOLOMICS: METHODS AND PROTOCOLS, Humana Press Inc, Totowa, 2019: pp. 69–95. https://doi.org/10.1007/978-1-4939-9690-2_5.
Ramaswamy, J.W. Hooker, R.S. Withers, R.E. Nast, W.W. Brey, A.S. Edison, Development of a ¹³C-optimized 1.5-mm high temperature superconducting NMR probe, J. Magn. Reson. 235 (2013) 58–65. https://doi.org/10.1016/j.jmr.2013.07.012.

Contact

Matt Merritt (Gainesville)
Ilya Litvak (Tallahassee)

Last modified on 20 November 2024

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