Electron Capture Dissociation

Neutralization-reionization mass spectrometry (MS) is of unique value for preparing and characterizing highly reactive and unstable neutral species.

These include the intermediate in the dissociative-recombination reaction H3O+ + e- → H2O + H + 6.4 eV. Following an earlier suggestion, using neutralization accompanying surface-induced dissociation (SID) to form an unstable site did not yield new cleavage reactions in multiply charged protein cations from electrospray ionization (ESI) with Fourier transform (FT) MS.

 

ECD ionization illustration

 

Citation

B. A. Cerda, et al, Electron Capture Dissociation of Multiply Charged Proten Cations. A Nonergodic Process, Journal of the American Chemical Society 120, 3265-3266 (1998) Read online  .

Relevant Publications

Y. Mao, et al, Top-Down Structural Analysis of an Intact Monoclonal Antibody by Electron Capture Dissociation-Fourier Transform Ion Cyclotron Resonance-Mass Spectrometry, Analytical Chemistry 85(9) pp 4239-4246 (2013) Read online .

This technique can be used with the following instruments:


For more information please contact Amy McKenna, Manager, ICR User Program.

Last modified on 7 June 2021