Fragmentation Techniques

These include Collision-Induced Dissociation, Electron Capture Dissociation, Electron Transfer Dissociation, Infrared Multiphoton Dissociation and Tandem Mass Spectrometry (MS/MS).

Collision Induced Dissociation (CID) or Collisionally Activated Dissociation (CAD) is commonly used to fragment gas-phase ions in mass spectrometry, especially from large, non-volatile molecules such as proteins.

Neutralization-reionization mass spectrometry (MS) is of unique value for preparing and characterizing highly reactive and unstable neutral species.

Electron transfer dissociation is a popular peptide fragmentation technique in mass spectrometry. It requires multiply-charged gas-phase cations (z>2) and therefore it is typically limited to an electron spray ion source.

Infrared multiphoton dissociation (IRMPD) is often used in conjunction with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) to fragment gas-phase ions inside the ICR cell.

Tandem MS (or MS/MS, MSn) is a technique to break down selected ions (precursor ions) into fragments (product ions). The fragments then reveal aspects of the chemical structure of the precursor ion.