FT-ICR Mass Spectrometry Enables Peptide de novo Sequencing
We describe a method for de novo protein sequencing with high accuracy and multiple levels of confidence. Samples are digested separately by two proteases, Lys-C and Lys-N. The resulting complementary pairs of ions combine to improve confidence in the identification.
- Research Area: Biochemistry, Biology
- Research Initiatives: Life
- Facility / Program: ICR
- Year: 2015