ICR Science Highlights
The Blood Proteoform Atlas: A reference map of proteoforms in human blood cells
A new Blood Proteoform Atlas maps 30,000 unique proteoforms as they appear in 21 different cell types found in human blood. The MagLab's 21 tesla FT-ICR mass spectrometer contributed nearly a third of the atlas' proteoforms.
Sunlight converts plastics into diverse chemical mixtures
Sunlight can chemically transform plastics from consumer plastic bags into complex chemical mixtures that leach into the ocean. Understanding the impact of plastic pollution requires advanced analytical techniques that can identify transformed plastic molecules in water samples, and requires instrumentation only available at the Maglab.
Makeup of Dissolved Organic Matter in Arctic Rivers
Researchers share new insights on the role of seasonality in dissolved organic matter (DOM) composition in large Arctic rivers.
ICR FAIR Data Improves Understanding of Protein Fragmentation
Reuse of the MagLab's Ion Cyclotron Resonance facility data improved understanding of protein fragmentation and aided the design and release of new algorithms and software tools. This is representative of a new type of MagLab user: A Data User – who accesses MagLab data from public data repositories to advance independent research goals.
Sunlight Produces Water-Soluble Chemicals from Asphalt
Road asphalt is made from aggregate (rocks) mixed with a "binder” from the residue remaining after extraction of gasoline and oils from petroleum crude oil. Until recently, this binder was thought to be chemically unreactive. Maglab scientists subjected a thin film of asphalt binder to simulated sunlight in the laboratory and used ultrahigh resolution mass spectrometry to reveal thousands of new, water-soluble chemicals that could be released into the environment by rainfall.
Prefractionation of Intact Proteins for Mass Spectrometry
Analysis of intact proteins using mass spectrometry is a difficult task that can be simplified by prefractionation, a process in which protein mixtures are separated into simpler fractions based on size. Here, researchers developed a new method, PEPPI-MS, which uses low-cost materials and common lab equipment to make an important protein separation strategy widely available.
Ultrahigh Performance Molecular Imaging using the 21T ICR Magnet
Combining spatial imaging technology with ultrahigh performance FT-ICR mass spectrometry provides users with the unique ability to create tissue images of identified biomolecules. This technology will be applied to understand human health and disease.
Corrosion Analysis on Acidic Crude Oils using FT-ICR
A new method to characterize crude oil corrosion shows that corrosion in acidic crude oils depends on the specific structures of the acid molecules, information that can help improve oil valuation and refining.
Identification of abnormal hemoglobin from human blood
Precise determination of hemoglobin sequence and subunit quantitation from human blood for diagnosis of hemoglobin-based diseases.
Researchers demonstrate method for analyzing molecules from bacteria cell walls
Scientists will be able to apply the technique to characterize similar molecules, helping develop vaccines and drugs to treat bacterial infection.
Functionalizing molecular nanocarbon with fluorine atoms
Researchers have discovered a new method to create encapsulated carbon nanomaterials that contain fluorine. Known as fullerenes, these nanocages are promising candidates for clean energy applications.
Ancient porphyrins indicate far earlier date for photosynthesis
Molecular fossils of chlorophyll (called porphyrins) more than 1.1 billion years old find suggest that photosynthesis began 600 million years earlier than previously established.
Targeted annotation of peptides by selective infrared multiphoton dissociation mass spectrometry
Protein oxidative damage is a common occurrence in a number of diseases, including cancer, neurodegenerative, and cardiovascular disease. Yet, little is known about its contribution to these illnesses. We developed a new technique, utilizing an infrared laser in combination with a mass spectrometer, to selectively identify sites of oxidation in complex protein mixtures. This sensitive and rapid platform may outperform current techniques and thus shed light on the involvement of oxidative damage in each of these diseases.
More accurate diagnosis for multiple myeloma
New technique could lead to precise, personalized cancer diagnosis and monitoring.
Decoding the human proteome with powerful mass spectrometer
The MagLab’s 21-tesla FT-ICR magnet can identify human proteins far more efficiently than commercial instruments, a boon for medical research.
MagLab develops, shares chemical analysis software
The high-tech tools empower scientists studying petroleum and other molecules to make decisions based on advanced data analysis.
A new twist on DNA
Research sheds important light on the fundamental process of cell division.
Removing "water-loving" molecules from petroleum
Scientists have developed a way to isolate emulsion-causing petroleum compounds. The technique may help lower energy costs for both oil companies and consumers.
Protein modifications in human breast cancer
We have discovered biomarkers that make it possible to distinguish breast cancer cells from non-cancerous cells, based on identifying chemical modifications of histones, the molecules about which DNA strands are wound to keep them in the cell nucleus. The method uses a high-field magnet to spread out the signals from different parts of the histone, to locate the site(s) of chemical modifications.
FT-ICR Mass Spectrometry Enables Peptide de novo Sequencing
We describe a method for de novo protein sequencing with high accuracy and multiple levels of confidence. Samples are digested separately by two proteases, Lys-C and Lys-N. The resulting complementary pairs of ions combine to improve confidence in the identification.
