ICR Science Highlights
A new Blood Proteoform Atlas maps 30,000 unique proteoforms as they appear in 21 different cell types found in human blood. The MagLab's 21 tesla FT-ICR mass spectrometer contributed nearly a third of the atlas' proteoforms.
Sunlight can chemically transform plastics from consumer plastic bags into complex chemical mixtures that leach into the ocean. Understanding the impact of plastic pollution requires advanced analytical techniques that can identify transformed plastic molecules in water samples, and requires instrumentation only available at the Maglab.
Researchers share new insights on the role of seasonality in dissolved organic matter (DOM) composition in large Arctic rivers.
Reuse of the MagLab's Ion Cyclotron Resonance facility data improved understanding of protein fragmentation and aided the design and release of new algorithms and software tools. This is representative of a new type of MagLab user: A Data User – who accesses MagLab data from public data repositories to advance independent research goals.
Road asphalt is made from aggregate (rocks) mixed with a "binder” from the residue remaining after extraction of gasoline and oils from petroleum crude oil. Until recently, this binder was thought to be chemically unreactive. Maglab scientists subjected a thin film of asphalt binder to simulated sunlight in the laboratory and used ultrahigh resolution mass spectrometry to reveal thousands of new, water-soluble chemicals that could be released into the environment by rainfall.
Analysis of intact proteins using mass spectrometry is a difficult task that can be simplified by prefractionation, a process in which protein mixtures are separated into simpler fractions based on size. Here, researchers developed a new method, PEPPI-MS, which uses low-cost materials and common lab equipment to make an important protein separation strategy widely available.
Combining spatial imaging technology with ultrahigh performance FT-ICR mass spectrometry provides users with the unique ability to create tissue images of identified biomolecules. This technology will be applied to understand human health and disease.
A new method to characterize crude oil corrosion shows that corrosion in acidic crude oils depends on the specific structures of the acid molecules, information that can help improve oil valuation and refining.
Precise determination of hemoglobin sequence and subunit quantitation from human blood for diagnosis of hemoglobin-based diseases.
Scientists will be able to apply the technique to characterize similar molecules, helping develop vaccines and drugs to treat bacterial infection.
Researchers have discovered a new method to create encapsulated carbon nanomaterials that contain fluorine. Known as fullerenes, these nanocages are promising candidates for clean energy applications.
Molecular fossils of chlorophyll (called porphyrins) more than 1.1 billion years old find suggest that photosynthesis began 600 million years earlier than previously established.
Protein oxidative damage is a common occurrence in a number of diseases, including cancer, neurodegenerative, and cardiovascular disease. Yet, little is known about its contribution to these illnesses. We developed a new technique, utilizing an infrared laser in combination with a mass spectrometer, to selectively identify sites of oxidation in complex protein mixtures. This sensitive and rapid platform may outperform current techniques and thus shed light on the involvement of oxidative damage in each of these diseases.
New technique could lead to precise, personalized cancer diagnosis and monitoring.
The MagLab’s 21-tesla FT-ICR magnet can identify human proteins far more efficiently than commercial instruments, a boon for medical research.
The high-tech tools empower scientists studying petroleum and other molecules to make decisions based on advanced data analysis.
Research sheds important light on the fundamental process of cell division.
Scientists have developed a way to isolate emulsion-causing petroleum compounds. The technique may help lower energy costs for both oil companies and consumers.
We have discovered biomarkers that make it possible to distinguish breast cancer cells from non-cancerous cells, based on identifying chemical modifications of histones, the molecules about which DNA strands are wound to keep them in the cell nucleus. The method uses a high-field magnet to spread out the signals from different parts of the histone, to locate the site(s) of chemical modifications.
We describe a method for de novo protein sequencing with high accuracy and multiple levels of confidence. Samples are digested separately by two proteases, Lys-C and Lys-N. The resulting complementary pairs of ions combine to improve confidence in the identification.