The recent resurgence of EPR in structural biology is in large part due to the development of pulsed EPR techniques and their applications in biological systems, especially distance measurements - PELDOR. PELDOR, also known as DEER (Double Electron Electron Resonance), has been demonstrated as a powerful tool for studying the topology and associated structural changes in proteins and nuclear acids. PELDOR is sensitive to spin-spin distance from 15 to ~100 Å, which is suitable to study large protein complexes. The EMR facility at the MagLab is equipped with two pulsed spectrometers that are capable to perform PELDOR experiments at 9.5 and 95 GHz.
Images & Sample Data
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Sample data.
Sample data.
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Distance measurements using pulsed EPR.
Distance measurements using pulsed EPR.
https://nationalmaglab.org/user-facilities/emr/emr-measurement-techniques/peldor#sigProId31a8bef859
Instrumentation
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Vileno, B, et al, Broad disorder and the allosteric mechanism of myosin II regulation by phosphorylation. P. Natl. Acad. Sci. U.S.A.,108 (2011) Read online
Song L, et al, Distance and dynamics determination by W-band DEER and W-band ST-EPR. Eur Biophys J. (2010) Read online
Fajer PG, et al, Practical Pulsed Dipolar ESR (DEER), Chapter 4 - ESR Spectroscopy in Membrane Biophysics, 27:95-128, ISBN: 978-0-387-25066-3 (2007) Read online