The recent resurgence of EPR in structural biology is in large part due to the development of pulsed EPR techniques and their applications in biological systems, especially distance measurements - PELDOR. PELDOR, also known as DEER (Double Electron Electron Resonance), has been demonstrated as a powerful tool for studying the topology and associated structural changes in proteins and nuclear acids. PELDOR is sensitive to spin-spin distance from 15 to ~100 Å, which is suitable to study large protein complexes. The EMR facility at the MagLab is equipped with two pulsed spectrometers that are capable to perform PELDOR experiments at 9.5 and 95 GHz.
Images & Sample Data
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- Sample data. Sample data.
- Distance measurements using pulsed EPR. Distance measurements using pulsed EPR.
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