Search results (74)

Name Description
Cell phone technology makes for versatile NMR probes

Inspired by the SIM card technology used in modern cell phones, MagLab engineers designed and built a versatile magnet probe that makes it easier and more efficient for scientists to see the structure of molecules.

Protein modifications in human breast cancer

We have discovered biomarkers that make it possible to distinguish breast cancer cells from non-cancerous cells, based on identifying chemical modifications of histones, the molecules about which DNA strands are wound to keep them in the cell nucleus. The method uses a high-field magnet to spread out the signals from different parts of the histone, to locate the site(s) of chemical modifications.

A Decade of Science on the Ultra-Wide-Bore 900 MHz NMR Spectrometer

Ten years ago the 900 Ultra-Wide Bore magnet became available to an international user community for Nuclear Magnetic Resonance spectroscopy and Magnetic Resonance Imaging at the National High Magnetic Field Lab. Since then 69 publications have been published from this instrument spanning many disciplines and the number of publications per year continues to increase with 26 in just the past 18 months demonstrating that state of the art data continues to be collected on this superb magnet.

FT-ICR Mass Spectrometry Enables Peptide de novo Sequencing

We describe a method for de novo protein sequencing with high accuracy and multiple levels of confidence. Samples are digested separately by two proteases, Lys-C and Lys-N. The resulting complementary pairs of ions combine to improve confidence in the identification.

Magnetic Resonance Imaging of an intact fruit fly brain at 10 micron resolution

In this paper, we obtained the first brain map of a complete fruit fly head at 10 micron isotropic resolution, the highest ever reported by MR for a complete head. Using two complementary imaging sequences revealed the superior power of DWI to dissect the brain architecture at close to cellular resolution.

An approach for dynamic nuclear polarization of membrane proteins

Dynamic nuclear polarization (DNP) coupled with solid state NMR can provide orders of magnitude enhancement to normally weak NMR signals, thereby enabling the study of inherently dilute proteins such as membrane proteins. Here we demonstrate a new approach to obtain DNP signal enhancements of membrane proteins by utilizing spin labeled lipids as the polarization agents. This strategy results in more than 2x in signal enhancements of a membrane protein when compared to standard DNP sample preparation techniques.

First Structure of a Protein in the M. tuberculosis Cell Division Apparatus

CrgA, a key Mycobacterium tuberculosis cell division protein that recruits five other proteins to the cell division apparatus has been structurally characterized using oriented sample and magic angle spinning solid state NMR. The protein has two transmembrane helices and an intrinsically disordered N-terminus. Binding sites have been identified for it's binding partners. Evaluating these binding sites may lead to effective drugs for either promoting and inhibiting cell division, both of which are of prime interest for the treatment of tuberculosis.

13C NMR Metabolomics: Applications at Natural Abundance

13C NMR when used in metabolomics 1. Provides better peak list for database matching and spectral annotation, 2. Provides better group separation and loadings annotation when using multivariate statistical analysis, and 3. Prevents possible misidentification of metabolites.

Detailed Spectroscopic Study of a Square Planar High-Spin FeII Complex

Square-planar high-spin Fe(II) molecular compounds are rare. Using an easily modifiable pincer-type ligand, the successful synthesis of the first compound of this type that breaks the FeO4 motif was achieved, and the first spectroscopic evidence that the geometry and spin state persist in solution was obtained.

Metabolic properties in stroked rats revealed by relaxation-enhanced MR spectroscopy at 21.1 T

By coupling selective band excitation of metabolites with high magnetic fields, relaxation-enhanced 1H MR spectroscopy can be performed in living specimen and patients to achieve high sensitivity over very short acquisition times for the examination of cellular dysfunction. This sensitivity can be used to evaluate otherwise inaccessible metabolites or regions of the proton spectral regime and can be used to probe cell-specific environments, such as neurons versus astrocytes, that may undergo differential changes during dysregulation.

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